ImmunoblottingCAT:
Cat. No. EHU048671: Western blot analysis of Catalase in HeLa cells.
Lanes1. HeLa cells transfected with Renilla Luciferase esiRNA (RLUC,
Cat. No. EHURLUC) as negative control.
2. HeLa cells transfected with CAT esiRNA (
Cat. No. EHU048671). Quantitative-Immunoblotting: Lysates were separated on SDS-Page and probed with mouse Monoclonal Anti-Catalase (1:2000) Clone: CAT-505 (
Cat. No. C0979) and goat Anti-GAPDH (1:7000) (Acris) antibodies. The signal intensities of indicated bands were quantified using an Odyssey infrared imaging system (LiCOR) and normalized to the GAPDH signal. At 72h post transfection, an 84% CAT protein reduction was measured between the negative control RLUC and the sample treated with CAT esiRNA.